Decolourization of Direct Blue 2 by peroxidases obtained from an industrial soybean waste


  • E Miranda-Mandujano Universidad Nacional Autónoma de México, Postgraduate Program in Environmental Engineering, Av. Universidad #3000, 04510, Distrito Federal México
  • G Moeller-Chávez Universidad Politécnica del Estado de Morelos, Environmental Engineering Department, Boulevard Cuauhnáhuac #566, Col. Lomas del Texcal, Jiutepec, 62550 Morelos, México
  • O Villegas-Rosas Benemérita Universidad Autónoma de Puebla, Postgraduate Program in Environmental Science, 14 sur #6301, Jardines de San Manuel, 72570 Puebla, México
  • Germán Buitrón Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Blvd. Juriquilla #3001, 76230 Querétaro, México
  • MA Garzón-Zúñiga Instituto Politécnico Nacional. Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR) Unidad Durango, México



decolourization, Direct Blue 2, industrial waste, soybean peroxidase, oxidation, toxicity


In this work the decolourization of Direct Blue 2 dye (DB2) using an industrial soybean waste as a source of peroxidases was studied. Temperature, pH, amount of H2O2 and concentration of dye were evaluated to determine the maximum catalytic activity of the enzyme during the dye degradation process. It was observed that a temperature of 40°C, a pH of 5 and a concentration of 40 mg/L for the dye in aqueous phase, play a significant role in the overall enzyme-mediated reaction. The maximum decolourization efficiency achieved under optimal conditions was 70% ± 4%. HPLC studies were carried out to confirm dye degradation and analyse the intermediate metabolites. The oxidation products quantified during the reaction were benzidine and 4 aminobiphenyl. Also, an increase in toxicity, determined by Vibrio fisheri, was observed after the enzymatic oxidation of the dye. Results suggest that the oxidation of DB2 with peroxidases can be recommended as a pretreatment step before a conventional treatment process.


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How to Cite

Miranda-Mandujano, E., Moeller-Chávez, G., Villegas-Rosas, O., Buitrón, G., & Garzón-Zúñiga, M. (2018). Decolourization of Direct Blue 2 by peroxidases obtained from an industrial soybean waste. Water SA, 44(2 April).



Research paper